2016_Head & Neck COURSE BOOK

DIAGNOSIS OF HPV-RELATED LESIONS

The mere detection of HPV-DNA is not sufficient to establish causality in HNCs!!! Castellsagué et al, J Natl Cancer Inst (2016)

Nowadays no standard test…

PROS

CONS

IHC/ISH/PCR

routinely used as a cost-effective surrogate marker high specificity for detecting active viruses 97% sensi t ivi ty and relat ively inexpensive experimental settings because of high sensitivity

p16 overexpress ion may occur independently of HPV less sensitive when there are low viral copy numbers

p16 INK4a immunohistochemistry

DNA in situ hybridization (ISH)

RNAscope (ISH)

it does not identify whether the virus is transcriptionally active

HPV-DNA PCR

E6/E7 HPV-mRNA PCR

the gold standard

high costs

Buckley et al, Aust NZ J Surg (2015)

mercoledì 29 giugno 16