7th ICHNO Abstract book

7th ICHNO 7 th ICHNO Conference International Conference on innovative approaches in Head and Neck Oncology 14 – 16 March 2019 Barcelona, Spain __________________________________________________________________________________________ page 57

Preliminary data included 13 pts experiencing grade ≤2 OM (moderate toxicity) and 11 pts with grade ≥3 OM (severe toxicity) during the treatment course. Eighteen pts and 6 patients received postoperative (60-66 Gy with conventional fractionation, CF) and radical IMRT (70 Gy with CF), with (15 pts), respectively or without (9 pts) concurrent chemotherapy. The most common primary tumor sites were oropharynx and nasopharynx. All patients had III and IV stage diseases (according to VIIth TNM edition). Salivary microbiota collected at baseline and after treatment was analyzable for all pts. We first evaluated the overall sequencing runs to cover the expected alpha-diversity. We investigated how microbial populations differ in HNC pts with or without severe OM. Fibrobacteres, Actinobacteria, and Hyd24-12 bacteria differentiated pts with severe OM and pts with moderate OM at baseline (p<0.05), while the presence of Acidobacteria at the end of treatment were associated with higher OM (p<0.05). Conclusion These very preliminary results seem promising in indicating that patients with and without severe OM have different salivary microbiota profile, both at baseline and at the end of Treatment. PO-113 DW MRI as biomarker of response during RT for intermed/high risk SCC oropharynx: a feasibility study C. Paterson 1 , I. McCrea 2 , L. Hay 3 , S. Allwood-Speris 4 , L. Devlin 3 , M. Sankaralingam 5 , P. McLoone 1 , C. Wilson 1 , D. Grose 1 , A. James 1 , C. Lamb 1 , M. Rizwanullah 1 , S. Schipani 1 , N. Ioanna 1 , M. Thomson 3 , M. McJury 4 , J. Foster 4 , A. Duffton 3 1 Beatson WoSCC, Clinical Oncology, Glasgow, United Kingdom; 2 NHS Greater Glasgow and Clyde, Radiology, Glasgow, United Kingdom; 3 Beatson WoSCC, Therapeutic radiography, Glasgow, United Kingdom; 4 NHS Greater Glasgow and Clyde, Medical Physics, Glasgow, United Kingdom; 5 Beatson WoSCC, Medical Physics, Glasgow, United Kingdom Purpose or Objective Despite radical chemo-radiotherapy (CRT), many patients with intermediate and high risk SCC oropharynx (OPSCC) relapse. Treatment related toxicity limits further uniform intensification across the patient group. If a predictive biomarker for outcomes from CRT can be identified during treatment, individualised and adaptive treatment strategies may be employed for the non-responders. This is the 1 st study to use DW MRI for early response assessment in a specific H&N sub-site with sub-type of similar biological behaviour and largest study using DW MRI as a predictive biomarker in H&N SCC generally. The primary aim of this study was to determine the feasibility of: Poster: Imaging and radiomics

complement component C7 were significantly higher in p16-DNA- compared with p16+DNA+ tumours (AUC 0.89

and 0.89). Conclusion

Our pilot study was able to identify several serum proteins that differentiated between HPV DNA-positive and DNA- negative OPSCC, and could thus potentially be used, in addition to p16 and/or HPV DNA detection, as a liquid biopsy to help stratify whether the OPSCC is HPV positive or negative. Efforts should be made to validate these findings in a larger cohort. PO-112 Role of microbiota in predicting oral mucositis in head and neck cancer patients treated with IMRT E. Orlandi 1 , N.A. Iacovelli 1 , L. De Cecco 2 , T. Rancati 3 , A. Cavallo 4 , L. Ferella 1 , E. Mancinelli 2 , M.S. Serafini 2 , A. Devecchi 2 , T. Giandini 4 , A. Cicchetti 3 , P. Bossi 5 , S. Alfieri 5 , L. Locati 5 , L. Licitra 6 , C. Fallai 1 , R. Valdagni 7 1 Fondazione IRCCS Istituto Nazionale dei Tumori, Radiation Oncology Unit 2, Milan, Italy; 2 Fondazione IRCCS Istituto Nazionale dei Tumori, Platform of Integrated Biology- Department of Applied Research and Technology Development, Milan, Italy ; 3 Fondazione IRCCS Istituto Nazionale dei Tumori, Prostate Cancer Program, Milan, Italy; 4 Fondazione IRCCS Istituto Nazionale dei Tumori, Medical Physics Unit, Milan, Italy; 5 Fondazione IRCCS Istituto Nazionale dei Tumori, Head and Neck Medical Oncology Department, Milan, Italy; 6 Fondazione IRCCS Istituto Nazionale dei Tumori, Head and Neck Medical Oncology Department- University of Milan, Milan, Italy; 7 Fondazione IRCCS Istituto Nazionale dei Tumori, Radiation Oncology Unit 1- Prostate Cancer Program- University of Milan, Milan, Italy Purpose or Objective Recently, dysbiosis of oral mucosal microbiota has been shown to potentially contributes to exacerbating oral mucositis (OM) severity in nasopharyngeal cancer patients (pts) undergoing RT. Thus, oral microbiota pattern could be considered one of the underlying dose-modifying factors, in addition to genetics and inflammatory microenvironment. The role of saliva/gut microbiota in driving radio-induced toxicity is under investigation in an Italian prospective trial aimed at providing new insight about the response of healthy tissues to radiotherapy (RT) for Head and neck (HNC) and prostate cancers (MICRObiota, infLammatory Environment, clinicAl and Radiomic features as predictors of Normal tissue response in radiotherapy for prostate; MICRO-LEARNER). Preliminary data for HNC patients (pts) are here presented. Material and Methods One-hundred thirty consecutive HNC pts receiving Intensity Modulated RT (IMRT) with or without chemotherapy at our Institute were enrolled in the discovery cohort. Clinical/dosimetric evaluation of patients’ features were performed pre-treatment, during RT and at treatment end; microbiota analyses were restricted at baseline and at treatment end. OM was classified according to CTCAE v4.0 scale. Salivary samples were collected using OMNIgene oral devices (DNA genotek). DNA extraction was carried out using the QIAamp-DNA-Kit (Qiagen). The bacterial 16S-ribosomal- RNA reads were analyzed with the QIIME-software and pooled in Operational Taxonomic Units (OTUs) with Uclust software. OTU data were used to calculate the index of biodiversity and principal component analysis was performed based on the relative abundance of OTUs. Results

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Recruiting patients

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Carrying out DW MRI at baseline (MRI 1) and week 3 (MRI 2) of RT

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Measuring apparent diffusion coefficient (ADC) on each scan for each target lesion, as per study protocol (Paterson et al, Clinical and translational radiation oncology, Feb 2017, Vol 2, p 13-18