Abstract Book

S916

ESTRO 37

SPSS statistics version 22.0 software package (SPSS Inc., Chicago, IL, USA). Results Median age was 55yrs (range 26-79yrs). The study included 78 patients with gynecological malignancies and 39 patients with breast cancer. Eight six percent received chemotherapy, 87% were subjected to radiotherapy. Median BMI was 23.88 kg/m 2 (16.4-36.4 kg/m 2 ), median weight was 55 Kg(35-83Kg) , median height was 151 cms (131-166cms). Median performance status was 80 (range 60-100). Sixteen percent of the patients routinely enrolled themselves in brisk walking >30minutes per day. T score spine was median -2.7(range 1.3 to -5.9), Z score spine median -1.6(range 1.6 to -4.3), median BMD spine 0.76 (range 0.40-58.05) and median BMC spine was 38.39(range 19.12-69.65). T score femur neck was median -1.9 (range 1.9 to -4.7), Z score femur neck median-0.8 (range 6.0 to -3.2), BMD femur neck median 0.67 (range 0.30-3.8) and median BMC femur neck was 3.13(range 0.83-41.04). Sixty one per cent of cancer survivors had osteoporosis, 30% had osteopenia and 9% had normal bone health. Factors significantly associated with osteoporosis included weight <50kg (p=0.001), KPS 70 or less (p=0.004), non breast cancer primary In the study 91% of patients had impaired bone health. Three out of five survivors had osteoporosis and 1 out of 3 had osteopenia. Factors significantly associated with osteoporosis included weight <50kg, KPS ≤70 and non breast cancer primary. EP-1708 Clinical relevance of DNA-damage response in metastatic breast and lung cancer under irradiation Y. Goy 1 , S. Riethdorf 2 , K. Rothkamm 3 , C. Petersen 1 , A. Krüll 1 , K. Pantel 2 , H. Wikman 2 , K. Borgmann 3 1 University Medical Center Hamburg-Eppendorf, Department of Radiotherapy and Radio-oncology, Hamburg, Germany 2 University Medical Center Hamburg-Eppendorf, Department of Tumor Biology, Hamburg, Germany 3 University Medical Center Hamburg-Eppendorf, Laboratory of Radiobiology & Experimental Radio- oncology, Hamburg, Germany Purpose or Objective Circulating tumor cells (CTCs) represent the first step of the metastatic cascade. In breast and lung cancer, high numbers of CTCs correlate with a more aggressive disease and independently predict progression-free as well as overall survival. The monitoring of CTCs under chemotherapy showed a correlation of persistent CTCs and shorter survival. This indicates stronger defense mechanisms in the remaining CTCs. More efficient DNA repair capacity could contribute to such stronger resistance. The aim of this study is to identify relevant DNA damage response pathways in CTCs and peripheral blood lymphocytes under radiotherapy and their possible implications for the adjustment of future therapies. Material and Methods 87 blood samples of patients with bone/brain metastatic breast/lung cancer (n=19/24) were collected before, at the end of radiotherapy and at the first follow-up. The number of CTCs at the first follow-up was compared with clinical treatment response (e.g. MRI/CT and performance status). Enumeration and characterization of CTCs were done using the CellSearch®system. Apoptosis was measured in CTCs (in vivo irradiation) with the help of the M30 antibody in the CellSearch®system (p=0.021). Conclusion

and DNA damage repair analyzed by yH2AX and 53BP1 foci detection was investigated in CTCs (in vivo) and primary lymphocytes ( ex vivo irradiation) to monitor genomic instability and DNA repair capacity of primary as well as tumor derived cells. Results Changes in the number of CTCs under local radiotherapy were observed in 62% of the patients. In breast cancer patient an increase, no change or a clear reduction in the number of CTCs was detected in 8, 42 and 50% of the cases respectively. The impact on systemic effects and survival is under estimation. The few lung cancer patients with detectable CTCs (16% at baseline) showed shorter survival compared to CTC free patients. An increase in the percentage of M30-positive CTCs was only observed in a subgroup of patients. Also the DNA repair capacity after ex vivo irradiation of primary lymphocytes indicates differences in the analyzed patient cohort. Conclusion The results indicate that monitoring DNA repair in CTCs and primary lymphocytes under radiotherapy is already showing excellent potential for judging treatment response. An increase in the percentage of apoptotic cells suggests less effective DNA repair whereas patients with lower percentage of apoptotic CTCs might have higher repair capacity. This correlates with individual DNA repair capacity showing less effective DNA repair in CTCs and poor prognosis. At present sample collection is ongoing to obtain data for a minimum of 100 patients. EP-1709 An effectiveness evaluation of the palmar fascia irradiation of patients with Dupuytren’s disease T. Latusek 1 , L. Miszczyk 1 , G. Gierlach 2 , P. Zając 3 1 Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Radiotherapy, Gliwice, Poland 2 District Hospital of Orthopedics and Trauma Surgery, Hand Surgery, Piekary Śląskie, Poland 3 District Hospital of Orthopedics and Trauma Surgery, Pediatric Orthopedics and Trauma Surgery, Piekary Śląskie, Poland Purpose or Objective Dupuytren's disease is a fibroproliferative disorder of an unknown etiology manifested by a progressive contracture of fingers. The basic method of the treatment is surgery. Among non-surgical treatments, radiotherapy (RT) represents a relevant method. The aim of the study was to evaluate the efficacy of palmar fascia irradiation in patients with Dupuytren's Disease. Material and Methods The research included a group of 117 patients with Dupuytren's disease irradiated in the Department of Radiotherapy in the Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology in Gliwice. Patients’ medical records from the Institute of Oncology in Gliwice and the Provincial Hospital of Orthopedics and Trauma Surgery in Piekary Slaskie have been analysed retrospectively. The following were assessed: smoking history and the subjective evaluation of the effect of the therapy and its side effects before irradiation and check-up visits. All patients were irradiated to a total dose of 21Gy given in 7 fractions. Results After the RT, 35% of patients showed an improvement, in 58% of patients the disease progress stopped, whereas 7% of patients reported a deterioration.