Brain Tumours

The 5-Gene Hh Signature

 The 5-gene Hh signature can robustly identify Hh-pathway activation in FFPE tumor samples by standard RT-PCR  Hh activation status determined by the 5-gene Hh signature is in 100% agreement with the Hh status determined by Affymetrix  A robust patient pre-selection tool for LDE225 treatment

GLI1 SHROOM2 SPHK1 PDLIM3

OTX2

Down-regulated

Up-regulated

Control genes: HUWE1, YME1L1, SOD1, LARP1

GLI1, glioma-associated oncogene homolog 1; HUWE1, HECT, UBA and WWE domain containing 1; LARP1, la ribonucleoprotein domain family, member 1;OTX2, orthodenticle homeobox 2; SHROOM2, shroom family member 2; SOD1, superoxide dismutase 1, soluble; PDLIM3, PDZ and LIM domain 3; SPHK1, sphingosine kinase 1; YME1L1, YME1-like 1; ZP2, zone pellucida sperm-binding protein 2. Amakye D, et al. AACR 2012. Abstract #4818.