ESTRO 2023 - Abstract Book

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ESTRO 2023

Materials and Methods The secretion of serum IL-2, IL-4, IL-6, IL-10, TNF, IFN- γ and IL-17a proteins in cervical cancer VMAT patients during radiotherapy were detected by Cytometric Bead Array (CBA) technology. The secretion of FOXP3, COX-2, TLRs, PD-1, PD- L1, EGFR and MAPK proteins were detected by Enzyme-Linked Immunosorbent Assays (ELISA). A co-culture system of fecal suspension from the cervical cancer patients during VMAT and human colorectal mucosal cells (FHC) cells were constructed. The protein expressions of I κκ , p-I κκ , NF- κ B-p65, IL-6 and COX-2 were detected by Western blot (WB). Results 1. It was found that in addition to IL-10, COX-2, TLRs, FOXP3, PD-1, PD-L1, EGFR, MAPK, IL-2, IL-4, IL-6, IFN- γ , TNF, and IL-17a molecules increased during VMAT compared with baseline through the determination of inflammatory factor proteins in peripheral blood, among which the secretion of COX-2, TLRs, IL-2 and IL-6 were time/dose dependent.

2. Through ROC curve analysis, it was found that IL-6 could be used as an inflammatory molecule to predict RE, and IL-6 and COX-2 could be used as inflammatory molecules to predict SARE. The inflammatory factors of RE and SARE were preliminarily identified. 3. The supernatant and cell lysate which were co-cultured with the fecal suspension from cervical cancer patients during VMAT and FHC were collected for total protein extraction. The protein expressions of p-I κκ , NF- κ B-p65, IL-6 and COX-2 were found to be time/dose dependent. Moreover, the phosphorylation of I κκβ at Ser176 and Ser177 up-regulated the expression of NF- κ B-p65, IL-6 and COX-2 downstream of the pathway.

Conclusion 1. The TLR signaling pathway was activated via gut microbiota after radiation. And the secretion of downstream IL-6 and COX-2 increased with radiation dose, both of which could be the inflammatory environmental factors to predict RE and