ESTRO 2023 - Abstract Book

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ESTRO 2023

Figure 1. Comparison of fits of our model and the IR model for dataset 69 in ref. 2 (left panel), and distribution of parameter pairs obtained for experimental datasets of ref. 2 (right panel) Conclusion The computational model presented here provides a quantitative framework linking mutation induction and low dose hyper- radiosensitivity and induced radioresistance. In contrast with the IR model, the parameters of our model have clear biological meaning and can be measured independently on the colony forming assay. However, it provides similarly good fit to experimental data. References 1. Polgár S, Schofield Paul N., Madas BG. 2022. Datasets of in vitro clonogenic assays showing low dose hyper-radiosensitivity and induced radioresistance. Sci Data 9(1):555. https://doi.org/10.1038/s41597-022-01653-3 2. Polgár S, Schofield Paul N, Madas BG. 2022. Data collection and analysis on low dose hyper-radiosensitivity and induced radio-resistance. https://doi.org/10.20348/STOREDB/1163 1 Medical University Vienna, AKH, Comprehensive Cancer Center, Department of Radiation Oncology, Vienna, Austria; 2 University Hospital Tuebingen, Department of Radiation Oncology, Tuebingen, Germany; 3 University Hospital Tuebingen, BG Unfallklinik, Department of Plastic and Reconstructive Surgery, Tuebingen, Germany; 4 University Hospital Tuebingen, Department of Orthopedic Surgery, Tuebingen, Germany; 5 University Hospital Tuebingen, Department of Pathology, Tuebingen, Germany Purpose or Objective High risk adult type soft tissue sarcoma are treated with multimodal concepts including surgery, radiotherapy and chemotherapy in selected cases. The prognosis is mainly limited by distant metastases, mainly in the lungs. In order to develop personalized treatment strategies (possibly including immunotherapeutic strategies) primary stem cell enriched cell lines were established in a prospective project and characterized in vitro. Materials and Methods Two established soft tissue sarcoma cell lines (SWS782, SWS 982, ATCC) were examined. In addition, as part of a prospective translational project, tissue samples of 11 soft tissue sarcomas were collected to establish stem-cell enriched cell cultures. mRNA abundances of different stem cell markers were characterised in four cell lines. Im-munogenic cell death marker PO-2223 Primary stem cell enriched sarcoma cell lines for radiobiological investigations F. Eckert 1,2 , J. Palm 2 , K. Ganser 2 , J. Kolbenschlag 3 , S. Sachsenmaier 4 , H. Boesmueller 5 , V. Potkrajcic 2