ESTRO 2024 - Abstract Book

S1732

Clinical - Lung

ESTRO 2024

Results:

As a result, low levels of baseline EV miR-375, miR-200c, and miR-30c were found in non-responders compared to responders. In particular, EV miR-30c identified non-responders with high accuracy showing an area under the curve of 87.2%. Low EV miR-30c and the presence of autophagic-activated CTCs were independent predictive biomarkers for shorter relapse-free survival and overall survival. Additionally, we observed that chemo- and radiotherapy increased levels of EVs miR-30c in in vitro models of treatment-induced autophagy and the administration of miR-30c, by direct transfection or encapsulated into human EVs, inhibited autophagy in those cells. This is the first report showing that EV miR-30c inhibits tumor autophagy and in combination with autophagic activated CTCs could be used as biomarkers for the stratification and monitoring of stage III NSCLC patients undergoing cCRT, who could also benefit from novel therapies based on autophagy inhibitors.

Conclusion:

Altogether, this study showed that EV miR-30c and autophagy CTCs are potential biomarkers for the prediction of the response to chemoradiotherapy and outcomes in locally advanced lung cancer patients. Hereby, we have demonstrated the important role of EVs miRNAs in regulating tumor autophagy and chemoradiotherapy resistance. These results could lay the first stone for using these liquid biopsy biomarkers in the stratification of patients that could also benefit from other combinatory therapies.

Keywords: Extracellular vesicles, autophagy, lung cancer,

References:

de Miguel Pérez D, Rodríguez Martínez A, Ortigodsa Palomo A, Delgado Ureña M, Gaercía Puche JL, Robles Remacho A, et all. Extracellular vesivle-miRNA as liquid biopsy biomarkers for disease identification and prognosis in metastatic colorectal cancer patients. Sci Rep. 2020; 10 (1): 3974

Huang DW, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatic resources. Nat Protoc. 2009; 4 (1): 44-57

Delgado Ureña M, Ortega FG, Miguel-Perez D, Rodriguez Martinez A, García Pucje JL, Ilyine H et all. Circulating tumor cells criteria (CyCAR) versus standard RECIST criteria for treatment response assessment in metastatic colorectal cancer patients. J Trans Med2018; (1)

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Effect of intervention timing of CRT combined with third generation EGFR-TKIs in EGFR-mutant NSCLC.

guangchuan deng, Zhenxiang Li