ESTRO 2024 - Abstract Book

S5252 ESTRO 2024 p<0.05 and 38.87 vs 2.675; p<0.05 respectively). LPS and irradiation increased ROS production, but GSE treatment mitigated these effects. Moreover, LPS significantly increased ROS production in Caco2 cells (12,9 vs 104; p<0.0001) and GSE treatment significantly prevented this damage (104 vs 11.3; p<0.0001). Irradiation increased ROS production in LPS-treated cells (3056 vs 362; p<0.0001) and also in this case GSE treatment was able to reduce ROS production due to irradiation (502 vs 357; p<0.0001). This beneficial effect was observed also in LPS treated cells and then exposed to irradiation (3056 vs 681; p<0.0001) (Figure 1). Radiobiology - Normal tissue radiobiology

Figure 1.

Conclusion:

GSE demonstrated the ability to protect intestinal epithelium from damage and reduce ROS production caused by LPS and ionizing radiation in an in vitro model. Further studies are needed to understand the mechanisms behind radiation-induced mucositis and GSE's antioxidant potential in prevention and clinical application.

Keywords: Mucositis, Grape, Extract

1235

Proffered Paper

Preclinical modeling of radiation cystitis and therapeutic strategy

Anne-Laure Pouliet 1 , Clément Brossard 1 , Morgane Dos Santos 2 , Mohamedamine Benajaoud 2 , Christelle Demarquay 1 , Valérie Buard 1 , Jean-Marc Simon 3 , Fabien Milliat 1 , Alain Chapel 1