ESTRO 2024 - Abstract Book

S5369

Radiobiology - Tumour biology

ESTRO 2024

This project intends to showcase the potential of on-chip MDT model for radiobiological studies. We demonstrated that RT effects can be recapitulated in MDTs and its combination with systematic drugs, such as senolytics, could be successfully tested on them. Moreover, ctDNA on chip may be a novel surrogate to measure cell death following treatments. Employing the this model in RT-related studies holds the potential to minimize the use of animal models in pre-clinical research. Furthermore, it could serve as a preferred tool in personalized medicine to identify optimal treatment approaches tailored to individual patients.

Keywords: Microfluidic chips, 3D tumour models, senolyitics

References:

1. Chermat, R. et al. Radiotherapy on-chip: microfluidics for translational radiation oncology. Lab Chip 10.1039.D2LC00177B (2022) doi:10.1039/D2LC00177B.

2. Nyga, A., Cheema, U. & Loizidou, M. 3D tumour models: novel in vitro approaches to cancer studies. J Cell Commun Signal 5, 239–248 (2011).

3. Astolfi, M. et al. Micro-dissected tumor tissues on chip: an ex vivo method for drug testing and personalized therapy. Lab Chip 16, 312–325 (2016).

4. Arulananda, S. et al. A novel BH3-mimetic, AZD0466, targeting BCL-XL and BCL-2 is effective in pre-clinical models of malignant pleural mesothelioma. Cell Death Discov 7, 122 (2021).

5. Hernandez-Segura, A., Nehme, J. & Demaria, M. Hallmarks of Cellular Senescence. Trends Cell Biol 28, 436–453 (2018).

6. Bousset, L. & Gil, J. Targeting senescence as an anticancer therapy. Molecular Oncology 16, 3855–3880 (2022).

7. Simeone, K. et al. Paraffin-embedding lithography and micro-dissected tissue micro-arrays: tools for biological and pharmacological analysis of ex vivo solid tumors. Lab Chip 19, 693–705 (2019).

8. Rostami, A. et al. Senescence, Necrosis, and Apoptosis Govern Circulating Cell-free DNA Release Kinetics. Cell Reports 31, 107830 (2020).

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Digital Poster

Potential of concomitant radiation therapy with gold nanoparticles for pancreatic cancer

Alexandra Martins 1 , Brigida C Ferreira 2 , Manuela Gaspar 3 , Sandra Vieira 4 , Joana Lopes 3 , Ana S Viana 5 , Antonio Paulo 6 , Filipa Mendes 6 , Paula Campelo 6 , Rui Martins 7 , Catarina Reis 2,3