ESTRO 2024 - Abstract Book

S5371

Radiobiology - Tumour biology

ESTRO 2024

At 72 h post-irradiation, cell viability for RT+HAOA-AuNPs at 50 μM was not significantly different from RT alone (p > 0.6). By contrary, in average a statistically significant loss in cell viability between RT+HAOA-AuNPs at concentrations of 200 μM and 400 μM and RT alone was obtained (p < 0.004), resulting in a loss in cell viability of 37%-51% compared to control. Also, for the 72 h post-irradiation time, RT+BBN-AuNPs at 50 µM and 200 µM induced a mean loss in cell viability of about 44% when compared to control (p < 0.0001) and significantly different than RT alone (p <0.0001). However, no statistically significant difference was obtained between RT+BBN-AuNPs at 200 µM and AuNPs alone. For this higher BBN-AuNP concentration, cell viability for the combined treatment seemed to be only caused by BBN-AuNPs.

Conclusion:

Conclusion: Post-irradiation incubation time is extremely relevant for the evaluation of cell viability by MTT assay. For the range of post-incubation times evaluated, the 72 h post-irradiation time proved to be most appropriate to evaluate the effects of RT. The combination of RT+HAOA-AuNPs at high concentration led to a similar cell viability compared to the RT+BBN-AuNPs at a low concentration demonstrating that the particle size and/or coating of tested nanoparticles influenced cell viability. Furthermore, the combination of RT with AuNPs led to cell viability loss significantly different from the control, or RT alone, thus representing a potential anticancer benefit. Additional in vitro studies, such as clonogenic assays, should be performed to support these findings.

Keywords: Pancreatic cancer, Gold nanoparticles, MV RT

1184

Digital Poster

Melittin acts as a radiosensitizer in non-small cell lung cancer by inducing apoptosis

Eunhui Seo 1 , Kyung-Ok Kim 2 , Kyeongah Kim 2 , Yunsook Choi 2 , Kyu Chan Lee 3

1 Department of Medicine, Gachon University College of Medicine, Incheon, Korea, Republic of. 2 Gachon Biomedical Convergence Institute, Gachon University Gil Medical Center, Incheon, Korea, Republic of. 3 Department of Radiation Oncology, Gil Medical Center, Gachon University College of Medicine, Incheon, Korea, Republic of

Purpose/Objective:

To investigate the potential radiosensitizing effect of melittin, a major component of bee venom, on non-small cell lung cancer (NSCLC). NSCLC is a prevalent and deadly form of lung cancer, with limited treatment options. Radiotherapy is a common treatment for locally advanced and unresectable NSCLC, but its effectiveness is often limited by cancer cell resistance. This study aimed to explore whether melittin could enhance the effectiveness of radiotherapy in NSCLC by promoting cell death and inhibiting DNA damage repair.

Material/Methods: