ESTRO 2024 - Abstract Book

S5423

Radiobiology - Tumour biology

ESTRO 2024

2. Pfister SX, Ahrabi S, Zalmas LP, Sarkar S, Aymard F, Bachrati CZ, Helleday T, Legube G, La Thangue NB, Porter AC, Humphrey TC. SETD2-dependent histone H3K36 trimethylation is required for homologous recombination repair and genome stability. Cell Rep. 2014 Jun 26;7(6):2006-18. doi: 10.1016/j.celrep.2014.05.026. Epub 2014 Jun 12. PMID: 24931610; PMCID: PMC4074340. 3. Edmunds JW, Mahadevan LC, Clayton AL. Dynamic histone H3 methylation during gene induction: HYPB/Setd2 mediates all H3K36 trimethylation. EMBO J. 2008 Jan 23;27(2):406-20. doi: 10.1038/sj.emboj.7601967. Epub 2007 Dec 20. PMID: 18157086; PMCID: PMC2168397.

2725

Digital Poster

Comparison of methods quantifying the effectiveness of Radiation Therapy with Gold Nanoparticles

Brigida C Ferreira 1 , Alexandra Martins 2 , Beatriz Galante 2 , Joana Dias 3 , Humberto Rocha 4 , Manuela M Gaspar 5 , Catarina Reis 1,5 1 Faculdade de Ciências da Universidade de Lisboa, Instituto Biofísica e Engenharia Biomédica, Lisbon, Portugal. 2 Faculdade de Ciências da Universidade de Lisboa, Física, Lisbon, Portugal. 3 Faculty of Economics, University of Coimbra, INESC Coimbra, Coimbra, Portugal. 4 Faculty of Economics, University of Coimbra, CeBER, Coimbra, Portugal. 5 Faculty of Pharmacy, Universidade de Lisboa, iMed.ULisboa, Research Institute for Medicines, Lisbon, Portugal

Purpose/Objective:

The delivery of nanoparticles of high atomic number during Radiation Therapy (RT) is a subject increasingly investigated. Gold Nanoparticles (GNP) are particularly interesting due to their high uptake by tumour cells, easy fabrication, biocompatibility, and low toxicity. Factors impacting the effectiveness of nanoparticles for therapy are their size, concentration, coating, etc, while for RT the beam energy and spectra are among the most studied factors. However, comparing findings from different studies is difficult due to the variety of methods used to quantify the effectiveness of the new treatment. Thus, in this study, methods generally used to evaluate the radiosensitizing effect of GNP on RT cell survival curves were estimated and compared considering the data reported in the literature.

Material/Methods:

Data from clonogenic assays reporting cell survival curves for RT alone and RT+GNP were collected from studies published in the literature. The experimental conditions of the performed assays were systematically documented and the results from clonogenic assays were retrieved using WebPlotDigitizer. Thirty-one studies published from 2010-2023 were found reporting data for the tumour cell lines: HeLa (22 assays), for breast cancer (MDA-MB-231(15), MCF-7 (5), MDA-MB-361 (2), T47D (2)), for prostate (PC-3 (10), DU-145 (6)), lung (A549 (7), L132 (2)), brain (U87 (4), T98G (3), U251 (2), F98 (2)), colorectal (HT-29 (2), LS180 (2)) and bladder (RT112 (1)). Cells, in the presence and absence of GNP, were irradiated with photon beams ranging from 50 kV-15 MV. Spherical GNP with sizes ranging from 1.9-120 nm, delivered with concentrations varying from 2.5 nM-5 mM, were used. A large diversity of coatings was tested