ESTRO 2024 - Abstract Book

S5427

Radiobiology - Tumour biology

ESTRO 2024

Figure 1 - Relative viability of PC3, fibroblasts and HEK cells for AuFe 25/75 and Au NPs (N=3).

Regarding the efficacy of the NPs in terms of enhancing the radiation effect, we observed an impact of different PEG coatings of Au NPs alone in the outcome, which we believed it is a result of different uptake by the cells and was later confirmed by ICP-MS analysis. In addition, Figure 2 shows that the addition of iron to the alloy, with gold, enhances even further the effect of radiation in PC3 tumour cells.

Figure 2 – Survival from clonogenic experiments after radiation: effect of iron in radiosensitizing PC3 cells (N=4).

Conclusion:

In this study we demonstrate in vitro the potential of Au and AuFe alloys in radiotherapy as radiosensitizers and contrast agents. They display low toxicity, with a preferentially higher tolerability in fibroblasts, contrast enhancement features and a radiosensitization effect in a prostate cancer tumour model. We also studied the role of ROS production and uptake of the NPs, where we observed the impact of particle size and coating type.