ESTRO 2025 - Abstract Book

S3945

Radiobiology - Normal tissue radiobiology

ESTRO 2025

Results: We successfully developed a functional, perfusable in-vitro microvascular network (Figure 2).

The data indicated an increase in the number of foci correlating with the dose (Figure 2). Over time, the number of DNA double-strand breaks (DSBs) decreased due to DNA repair mechanisms. Notably, repair dynamics differed between samples exposed to conventional photon and carbon ion irradiation. Cells exposed to carbon ions showed larger foci than those treated with photons, suggesting more densely clustered DSBs, which result in more complex, challenging-to-repair damage. Conclusion: We established a robust protocol for simulating carbon ion treatment on a chip, allowing for the in-vitro analysis of microvascular damage within a 3D environment. This study enables future on-chip models to serve as radiobiological tools for carbon ion radiation therapy research. The technology also paves the way for further analyses regarding microvascular wall permeability or cytokines concentrations[1,3], even including tumor cells[4] and dynamic phenomena[1,5,6].

Keywords: microvasculature, hadron therapy, lab-on-chip

References: (1)

Offeddu, G. S. et al. Small 2019 , 15 (46), 1902393. Possenti, L. et al. Cancers (Basel) 2021 , 13 (5), 1182. Guo, Z. et al. Adv Mater Technol 2019 , 4 (4), 1–12. Offeddu, G. S. et al. Advanced Science 2024 . Hajal, C. et al. Biomaterials 2021 , 265 , 120470. Rota, A. et al. Bioeng Transl Med 2023 , 8 (5).

(2) (3) (4) (5) (6)

Acknowledgments The AIRC Investigator Grant no. IG21479 supported this work.