ESTRO 2025 - Abstract Book

S3984

Radiobiology - Tumour radiobiology

ESTRO 2025

Results:

BB with 4 Gy and 6 Gy led to a significant increase in 2D migration of A549 which was not demonstrated after MRT (Fig 1A). For 3D migration, no significant increase was observed after BB but EUD to 4 and 6 Gy MRT led to a significant reduction (Fig 1B) showing the differential modulation of motility between BB and MRT. Different doses of BB led to a significant increase (up to 90% higher, p<0.05) in adhesion of the A549 cells compared to non irradiated cells and a significant increase in the expression of MMP9 (log2(FoldChange)=1.2, p<0.001), a crucial player on cell migration 3 . Finally, when in co-culture BB irradiation of MRC-5 stimulated the 3D migration of non irradiated A549 cells (p<0.01). Conclusion: This study is the first reporting the differential modulation of non-small cell LC motility after BB or MRT. The improvement on motility outcomes observed with MRT suggest its potential benefit on better controlling metastasis improving LC treatment. Further experiments are currently ongoing to better elucidate the mechanisms behind the different findings.

Keywords: metastasis, cell motility, spatial fractionation

References: 1. Zhao, Q. et al. Suppression of Radiation-Induced Migration of Non-Small Cell Lung Cancer through Inhibition of Nrf2-Notch Axis . www.impactjournals.com/oncotarget (2017). 2. Trappetti, V. et al. Synchrotron Microbeam Radiation Therapy for the Treatment of Lung Carcinoma: A Preclinical Study. Int J Radiat Oncol Biol Phys 111 , 1276–1288 (2021). 3. Merchant, N. et al. Matrix Metalloproteinases: Their Functional Role in Lung Cancer . (2017).

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Digital Poster 3D MedNext Model for the Automated Delineation of High Metabolic Regions in Nasopharyngeal Carcinoma on Computed Tomography Images Guanqun Zhou 1,2 , Zijie Mo 3 , Mengyu Hao 1,4,2 , Yuxian Yang 1,2 , Yuxi Xiong 1,2 , Hua Li 3 , Lecheng Jia 3 , Yida He 5 , Guangyu Wang 1,2 , Xiaobo Jiang 1,2 , Feng Chi 1,2 , Ying Sun 1,2