ICHNO-ECHNO 2022 - Abstract Book

S52

ICHNO-ECHNO 2022

geometry, obtained from MRI scans and cadaver anatomical reviews as well as human nerve models recently developed in the field of computational neuroscience.

In Vivo study

A pilot study was carried out on 7 healthy volunteers. We collected saliva over 5 minute periods with either, no stimulation (baseline), with the electrode stimulation, and with 1ml of lemon juice instilled on the tongue (positive control). A 15- minute interval was given between each collection Results Our novel bipolar concentric ring electrode design enables precise and focused stimulation of the auriculotemporal nerve while avoiding spillover to nearby structures. This small design makes for a more discrete wearable system. The concentric bipolar configuration penetrates deeper with the same current than existing TENS electrodes with the same diameter allowing for smaller currents to be used. This reduces skin current density and discomfort compared with previous TENS techniques. The pilot study revealed increased saliva production, relative to baseline levels following stimulation of the auriculotemporal nerve (p-value < 0.01). Conclusion Focused stimulation of the auriculotemporal nerve increases saliva production. This is a novel approach to treat xerostomia post radiation therapy. We used a computational model to design a novel electrode and demonstrated, in vivo, that it increases saliva secretion in healthy volunteers.

PO-0089 Feasibility and clinical outcome upon re-irradiation and deep hyperthermia in H&N cancer patients

Abstract withdrawn

PO-0090 Innovative combination of targeted therapies for thyroid cancer treatment

S. saussez 1 , L. Gheysen 1 , L. Soumoy 1 , F. Journe 2

1 University of Mons, Laboratory of human anatomy and experimental oncology, Mons , Belgium; 2 University of Mons,, Laboratory of human anatomy and experimental oncology, Mons , Belgium Purpose or Objective Our objective is to overcome resistance difficulties in thyroid cancer treatment and respond to a lack of efficient therapeutic strategy especially for the anaplastic cancer. We investigate the synergistic potential activity of two small molecules and combine dasatinib (multi-kinase inhibitor) and PRIMA-1Met (p53 reactivator). Materials and Methods Apoptosis, proliferation, migration and invasion assays have been performed on 6 thyroid cancer cell lines (two papillary forms (BCPAP, TPC-1), two folliculars (FTC-133, TT2609-CO2) and two anaplastic (8505C, CAL-62)). Combination index have been calculated with Calcusyn software (Biosoft, UK). Mechanisms implicated in targeted cellular pathways such as MAPK, PI3K, SRC or p53 have been studied by Western blot. Subcellular localization of proteins targeted by the treatment has been observed by immunofluorescence. Animal model has been used to assess the effect of combination on tumor volume inhibition. Results The evaluation of the pro-apoptotic properties of the combination demonstrated that dasatinib and PRIMA-1Met act in synergy to induce a significant increase in cell death by apoptosis as well as a decrease in cell proliferation. The dasatinib molecule also showed anti-migration and anti-invasive properties. A mechanistic study by Western blot on the anaplastic line 8505c allowed us to demonstrate an inhibition of SRC and AKT phosphorylations by the combination of treatments, but this has no effect on ERK phosphorylation. Finally, an orthotopic model of 8505c cells injected into immunosuppressed mice was developed. Daily administration of the combination of dasatinib and PRIMA-1Met produced a small but significant decrease in tumor volume. Conclusion Even if complementary experiments are necessary to fully understand the success of our combined treatment both in vitro and in vivo, our preliminary results demonstrate strong synergistic effects of dasatinib and PRIMA-1Met on different cell lines representative of the different forms of thyroid cancer and especially of the anaplastic one which is still incurable nowadays.

PO-0091 Effect of bio adhesive barrier forming oral liquid on radiation induced Oral mucositis HNSCC

A. Devalla 1 , L. Vishwanath 2 , N. Thiammiah 3 , S. Palled 3 , V. Cr 4 , S. Poojar 5 , A. Gv 6

1 kidwai Memorial Institute Of Onoclogy, Radiation Onoclogy, Bangalore, India; 2 kidwai Memorial Institute Of Onoclogy, Radiation Onoclogy, Radaitaion Onoclogy, Bangalore, India; 3 kidwai Memorial Institute Of Onoclogy, Radiation Onoclogy,