Research Masterclass in Radiotherapy Physics 2017
Reduced Growth and Viability of tumour spheroids following microbeam Irradiation.
• A549 Spheroids treated with Microbeam or Broadbeam Irradiation
Day 0 Day 7 Day 14
Control
• Growth measured using Celigo cytometer
• Viability measured at 14 days post treatment
18Gy
• The predicted response of the spheroids to microbeam irradiation was calculated based on the assumption of no communication between cells.
Dose [Gy]
100
• The actual viability following MB falls below the predicted
BB Survival Predicted MB survival
10
BB survival MB survival
• Cell:cell communication plays a role in MB response in 3D culture
Survival [%]
1
0
5
10
15
20
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