Dose Course 2018_Flipping book

Bystander and non-target irradiation effects on normal and radiosensitive lymphocytes

• Used normal and radiosensitive cell lines

– Irradiated in- and out of field 6MV

– +/- Media from one transferred to others

• Out-of-field radiation induces g-H2AX expression in radiosensitive cells

• Out of field radiation decreases growth of radiosensitive cells

• Out of field radiation and addition of irradiated cell conditioned media differentially affects cell line responses • Treatment of normal responding cells with conditioned media from cells of different radiosensitivities varies their cytokine gene expression pattern • Out of field radiation increases DNA damage in primary human peripheral blood mononuclear cells

• Conclude out of field radiation induces early DNA damage in radiosensitive cell lines and primary blood cells and reduces cell proliferation over 5 days.

– Implications for radiosensitive patients

• Important to determine out of field dose accurately and investigate effects.

J. Bryant, L. Shields, C. Hynes, M. Taylor, O. Howe, BMcClean, F. Lyng Submitted June 2018

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