ESTRO 2024 - Abstract Book

S5225

Radiobiology - Microenvironment

ESTRO 2024

Literature data for cytokine production by irradiated HUVECs spans several orders of magnitude (IL6: 0.01-10 pg/1k cells; IL8: 0.01-100 pg/1k cells), reaching concentrations of tens to hundreds of pg/ml [4–7]. Our results align with these ranges (IL6: 0.5-10 pg/1k cells; IL8: 0.5-1.5 pg/1k cells). These findings can be coupled with the permeability alteration after irradiation, with vessels exhibiting increased leakage 3 hours after irradiation with 2 and 5 Gy [3].

Conclusion:

We have presented a novel on-chip model for studying the effect of radiation-induced inflammation on the microvasculature. This technology enables the simultaneous evaluation of microvascular wall permeability and the cytokines released in the media within a three-dimensional relevant microenvironment. Our data support the alteration of cytokines by radiation, particularly IL6. The presented device can be used to specifically test the effect of the altered cytokines on vascular permeability (e.g., the effect of IL6 on junction proteins and permeability [8,9]).

Keywords: Lab-on-chip, inflammation, microvasculature

References:

1. De Ruysscher, D. et al. Radiotherapy toxicity. Nat Rev Dis Primers 5, 13 (2019).

2. Chen, M. B. et al. On-chip human microvasculature assay for visualization and quantification of tumor cell extravasation dynamics. Nat Protoc 12, 865–880 (2017).

3. Possenti, L. et al. OC-0094 Radiobiological models for microvascular damage including functional impairment of capillary wall. Radiotherapy and Oncology 182, S57–S58 (2023).