ESTRO 2024 - Abstract Book

S712

Clinical - Breast

ESTRO 2024

Rocio Benavides 1 , Cecilia Escuin Troncho 1 , Olga Engel 1 , David Parada 5 , Miriam De la flor 6 , Coia Jordà 7 , Mireia Melé 8 , Jana Repkova 8 , Meritxell Arenas Prat 1 1 Hospital Universitari Sant Joan de Reus, Radiation Oncology, Reus, Spain. 2 Universitat Rovira i Virgili, Unitat de recerca biomédica, Tarragona, Spain. 3 Institut d`investigació sanitaria Pere Virgili, Radiation Oncology, Tarragona, Spain. 4 Institut d`investigació sanitaria Pere Virgili, Unitat de recerca biomédica, Tarragona, Spain. 5 Hospital Universitari Sant Joan de Reus, Pathology, Reus, Spain. 6 Hospital Universitari Joan XXIII, Gynecology, Tarragona, Spain. 7 Hospital Universitari Sant Joan de Reus, Gynecology, Reus, Spain. 8 Hospital Universitari Sant Joan de Reus, Oncology, Reus, Spain

Purpose/Objective:

Breast cancer (BC) is a complex and heterogeneous disease, with different prognoses and different responses to oncological treatments. The interplay between oxidative stress and the inflammatory system plays a significant role in the onset and progression of the disease. Previous studies have determined that various interleukins are altered in BC patients, and these alterations can vary based on BC subtypes and clinicopathological features. The objective of this study is to comprehend the circulatory alterations in BC patients. To achieve this, we analyzed the levels of proteins related to oxidative stress and the immune system. Additionally, we determined whether these circulatory alterations correlated with clinicopathological characteristics.

Material/Methods:

We recruited 85 women with BC and compared them with 50 healthy women. Prior to any treatment, blood samples were collected to analyze various biochemical parameters such as glucose, cholesterol, and VLDL. Using ELISA and colorimetric analysis, we determined inflammatory proteins (CCL2, IL-10, and TGF- β) and oxidative stress markers (PON1).

Results:

Through multivariate analysis, statistically significant alterations were identified between BC patients and controls. In relation to the values of anti-inflammatory cytokines (increased IL-10 and TGF- β), elevated levels of glucose and lipid-related variables (total cholesterol, VLDL, and TG) were observed in BC patients. It was also observed that factors such as postmenopausal status, histological grade III tumors, and the triple negative molecular subtype were associated with oxidative stress alterations (reduced PON1) and inflammatory responses (increased IL-10 and TGF- β), as well as an increase in tumor markers (CEA and CA15.3).

Conclusion:

The study reveals a spectrum of alterations in the levels of proteins related to the immune response, anti inflammatory cytokines (IL-10 and TGF- β), and oxidative stress in BC patients. Furthermore, postmenopausal patients, high histological grade (III) tumors, and the triple-negative subtype were showed more oxidative stress and inflammation alterations.